TLR2 in γδ T cells is required for metabolic support of their IL-17A response to a commensal bacterium on the ocular surface

Abstract The ocular surface is colonized by commensal microbiota, which tune the local mucosal immune response. We previously identified an commensal, Corynebacterium mastitidis (C. mast), induced IL-17A production from γδ T cells in conjunctiva and eye-draining lymph nodes (DLN), enhanced host resistance to fungal bacterial pathogens. To elucidate how producing (γδT17) are activated C. mast, we associated WT TLR2−/− mice with investigated role of TLR2 this process. This was based on rationale that mastis a Gram-positive bacterium expression γδT increased after mast association. Mechanistic studies showed required not only DCs (for IL-1 production) but also cells. RNA-Seq revealed genes related mitochondrial oxidative phosphorylation were dysregulated γδT17 cells, indicating engagement may regulate metabolic processes Seahorse assay confirmed T17 displayed impaired oxygen consumption rates lower ATP production. Interestingly, lysosomal activity, plays central cellular metabolism, decreased Enhancement activity curcumin C1 restored generation propose intrinsic signaling for support their